Anti-MYL12A (phospho S18) Antibody

This rabbit polyclonal antibody from Antibodies.com is raised against myosin light chain 12A (MYL12A) phosphorylated at serine 18, a regulatory modification catalyzed by myosin light chain kinase (MLCK). The phospho-specific reagent discriminates the active, kinase-primed form of MYL12A from unphosphorylated light chain, supporting studies of actomyosin contractility, cytokinesis, cell migration, and smooth muscle mechanotransduction.

Validated for Western blot and immunohistochemistry, the unconjugated IgG reagent is supplied as a 200 µL volume and is reactive against human, mouse, and rat samples. The polyclonal format tolerates minor sequence variation across species, while the unconjugated format allows downstream pairing with HRP, fluorophore, or biotin secondary detection chemistries selected by the end user.

Typical working applications include immunoblotting of cell and tissue lysates to follow MLCK and Rho-kinase signaling, and IHC on formalin-fixed paraffin-embedded sections to localize contractile activity in vascular, intestinal, and tumor tissues. Store at -20 degrees C in working aliquots to limit freeze-thaw cycles and preserve phospho-epitope recognition.