Anti-MTR4 Antibody

MTR4 is a nuclear DExH-box RNA helicase that serves as the unwindase component of the TRAMP complex, channeling aberrant and unprocessed transcripts toward polyadenylation and exosome-mediated degradation in the nucleolus. This unconjugated Rabbit polyclonal reagent from Antibodies.com is raised against the endogenous MTR4 protein (also called SKIV2L2), supporting research on ribosome biogenesis, rRNA maturation, and nuclear RNA surveillance.

Each vial is supplied at 100 µl in a format ready for direct detection or downstream labeling. The antibody is validated for western blot and immunohistochemistry applications, giving consistent signals in human, mouse, and rat samples. Researchers can monitor endogenous MTR4 expression in whole-cell lysates by WB and visualize its characteristic nucleolar distribution by IHC. Store the vial refrigerated for short-term use; for long-term storage, freeze aliquots and limit freeze-thaw cycles to preserve binding activity.

Typical applications include probing TRAMP complex assembly on model pre-rRNA substrates, characterizing mutations that disrupt ribosomal RNA processing, and tracking how loss of MTR4 function alters exosome substrate flux in disease models. Cross-reactivity with rodent orthologs makes this reagent useful for comparative studies of RNA quality control between human cell lines and mouse or rat tissues.