Anti-mTOR (phospho S2448) Antibody

Produced in rabbit as a polyclonal IgG antibody, this phospho-specific reagent from Antibodies.com is raised against the mammalian Target of Rapamycin (mTOR) when phosphorylated at serine 2448. S2448 is one of the most studied activation marks on mTOR, primarily driven by Akt/PKB kinase activity, and is widely used as a read-out of upstream PI3K/Akt signaling in cell and tissue experiments.

Supplied as a 200 µl vial of unconjugated antibody, the reagent is validated for Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF) work on human samples. It is commonly used to study mTORC1/mTORC2 pathway flux, insulin and growth factor responses, autophagy regulation, and oncogenic signaling in cancers with hyperactive PI3K/Akt/mTOR pathways, including breast, prostate, and colorectal tumor models.

For best stability, store at -20°C in working aliquots and avoid repeated freeze-thaw cycles. Pairing this phospho-S2448 reagent with Antibodies.com antibodies against total mTOR or alternative phosphorylation sites such as S2481 lets users separate basal expression from pathway-driven activation in their readouts.