Anti-mTOR (P2476) Antibody

mTOR (mechanistic Target of Rapamycin) is a serine/threonine kinase that integrates growth factor, nutrient, and energy cues to govern cell growth, proliferation, autophagy, and cap-dependent protein translation. The P2476 residue sits in the kinase domain and serves as a readout of mTOR catalytic activity, making phospho-specific reagents against this site valuable for cancer biology, immunology, neuroscience, and metabolic disease research.

This rabbit polyclonal reagent is produced by Antibodies.com and is raised against a region encompassing P2476 of human mTOR, with demonstrated reactivity against mouse and rat orthologues for cross-species studies. It is supplied unconjugated at 1 mg/ml in phosphate buffered saline with 0.05% sodium azide at approximately pH 7.2, in a 100 µl vial, providing enough material for dozens of Western blot membranes or numerous immunohistochemistry runs depending on dilution.

Validation covers Western blot and immunohistochemistry applications. On Western blot, lysates prepared in standard RIPA or NP-40 buffers with protease and phosphatase inhibitors preserve the phospho-epitope, and detection is typically achieved with HRP- or fluorophore-conjugated secondary reagents. For IHC, antigen retrieval in citrate or EDTA buffer on formalin-fixed paraffin-embedded sections is the standard workflow, allowing visualization of pathway activation in tumor microarrays, brain sections, or insulin-responsive tissues such as liver, pancreas, and skeletal muscle. Researchers mapping the PI3K/AKT/mTOR axis frequently combine this antibody with reagents against phospho-S6K, phospho-4E-BP1, or total mTOR to resolve upstream and downstream signaling events.

Upon arrival, the vial should be refrigerated; for longer-term storage, prepare aliquots to limit freeze-thaw cycles, and observe standard precautions for sodium azide-containing solutions. The reagent is intended for research use only and is not approved for diagnostic or therapeutic applications.