Anti-mTOR Antibody

This rabbit polyclonal antibody recognizes the mechanistic Target of Rapamycin (mTOR), a ~289 kDa serine/threonine kinase that sits at the core of the PI3K/AKT/mTOR signaling axis and governs cell growth, proliferation, autophagy, and translational control. The reagent is affinity-purified to an IgG isotype and supplied unconjugated in a 200 µl vial, leaving it free for direct labeling or for pairing with HRP, biotin, or fluorophore secondary reagents in custom detection workflows.

Validated applications include western blot analysis of total and phosphorylated mTOR in cultured cells and tissue lysates, immunohistochemistry on formalin-fixed paraffin-embedded human biopsy sections to map mTOR expression across tumor and matched normal tissue, and immunofluorescence on fixed-cell preparations for subcellular localization studies. Reactivity is reported for human samples, supporting translational research programs in oncology, metabolic disease, and neuroscience where pathway dysregulation is implicated.

The polyclonal format captures multiple epitopes across the target, improving tolerance for partial proteolysis and post-translational modification in crude sample preparations. Each lot is manufactured by Antibodies.com as part of their primary antibody portfolio. For best activity, store at -20 °C in working aliquots to limit freeze-thaw cycles, and pair with appropriate blocking reagents and species-matched secondaries when designing western blot, IHC, or IF panels.