Anti-mTOR (Ab-2448) Antibody

Manufactured by Antibodies.com, this rabbit polyclonal reagent is raised against the mechanistic target of rapamycin (mTOR) and is designed to detect endogenous levels of the total mTOR protein. The antibody was generated by immunizing rabbits with a synthetic peptide corresponding to amino acids 2446–2450 (T-D-S-Y-S) of human mTOR, and it was affinity-purified by epitope-specific peptide chromatography to a final concentration of 1.0 mg/mL. Supplied as a 50 µl liquid aliquot in unconjugated form, it is formulated in phosphate-buffered saline (pH 7.4) containing 150 mM sodium chloride, 0.02% sodium azide, and 50% glycerol, and is intended for research use only in Western blot, immunohistochemistry, and immunocytochemistry/immunofluorescence (ICC/IF) workflows. The antibody has been validated for reactivity with human, mouse, and rat samples and is shipped at 4°C; upon arrival it should be aliquoted and kept at –20°C, with repeated freeze/thaw cycles avoided to preserve activity. Target synonyms listed by Antibodies.com include FRAP, FRAP1, FRAP2, RAFT1, RAPT1, and serine/threonine-protein kinase mTOR, reflecting its recognition of the conserved regulatory kinase domain region. This Antibodies.com polyclonal offers a flexible option for laboratories studying mTOR signalling, autophagy, and translational control across multiple model systems.