Anti-mTOR (Ab-2448) Antibody

Raised in rabbit as a polyclonal reagent, this unconjugated primary antibody from Antibodies.com targets the mechanistic target of rapamycin (mTOR), recognizing an epitope centered on residue 2448 of the kinase. The antibody is supplied as 100 µl of stock and is validated for western blotting, immunohistochemistry, and immunofluorescence workflows on human, mouse, and rat samples. mTOR is a roughly 289 kDa serine/threonine kinase in the PI3K-related family that integrates nutrient, growth factor, and energy cues to govern cell growth, autophagy, translation, and metabolism; phosphorylation at Ser2448, introduced by Akt/PKB downstream of insulin and IGF-1 signaling, is widely read as a hallmark of pathway activation. Laboratories commonly deploy this reagent to monitor PI3K/Akt/mTOR activity in tumor biology, metabolic disease, and neurodegeneration models, and to compare baseline versus stimulated signaling in treated versus control cohorts. As an unconjugated IgG preparation, it pairs freely with user-selected secondary antibodies and detection chemistries, including HRP, biotin, and a broad panel of fluorophores for multiplex imaging. For best stability, store at -20 °C in working aliquots and limit repeated freeze-thaw cycles; centrifuge briefly before use and handle only on ice to preserve activity across long-term studies.