Anti-MTAP Antibody

Raised in rabbit against the human methylthioadenosine phosphorylase (MTAP) protein, this unconjugated polyclonal primary antibody from Antibodies.com is supplied as 100 µl of ready-to-use reagent for Western blot and immunohistochemistry workflows. MTAP is a key enzyme in the methionine salvage and polyamine biosynthesis pathways, where it catalyzes the reversible phosphorolytic cleavage of 5'-methylthioadenosine into adenine and 5-methylthioribose-1-phosphate. Because the MTAP gene sits at chromosome 9p21, immediately adjacent to the CDKN2A locus, its homozygous deletion is frequently observed together with CDKN2A loss in gliomas, malignant pleural mesothelioma, pancreatic adenocarcinoma, non-small-cell lung cancer, and subsets of melanoma. Researchers therefore use MTAP immunostaining as a practical surrogate marker when screening tumor sections for 9p21 co-deletion, complementing FISH or sequencing-based confirmation.

The reagent is reactive with human MTAP and is suited to Western blot detection of the endogenous protein in cell lysates as well as IHC on formalin-fixed paraffin-embedded tissue sections, supporting studies of MTAP expression loss, salvage pathway activity, and tumor suppressor function. The unconjugated format allows downstream pairing with HRP-, biotin-, or fluorophore-conjugated secondary reagents of the researcher's choice, and the polyclonal nature of the reagent provides multi-epitope coverage for detecting MTAP even when expression is reduced. Store refrigerated and protect from light to preserve antibody activity across repeated applications.