Anti-MT-ATP6 Antibody

MT-ATP6 is one of the 13 proteins encoded by the human mitochondrial genome and forms subunit a of the membrane-embedded F0 sector of the F1F0 ATP synthase rotary motor. The complex catalyses the final step of oxidative phosphorylation, coupling proton re-entry across the inner mitochondrial membrane to ATP synthesis. Point mutations such as m.8993T>G are causal in NARP, maternally inherited Leigh syndrome, and related neurological and metabolic phenotypes characterised by impaired mitochondrial energy output, which is why the protein is a frequent target in studies of mitochondrial disease.

This unconjugated rabbit polyclonal reagent from Antibodies.com was raised against the MT-ATP6 target and is supplied as 50 µl of a 1 mg/ml stock in PBS containing 0.1% sodium azide and 50% glycerol. Validation has been carried out for western blot detection of the MT-ATP6 protein in lysates derived from human, mouse, and rat origins, supporting comparative work on mitochondrial bioenergetics across these three species in tissues, cultured cells, or isolated mitochondria. The unconjugated format allows laboratories to pair the primary with HRP- or fluorophore-conjugated secondaries for either chemiluminescent or fluorescent western blot readout.

For handling, store frozen at -20 °C in working aliquots to limit repeated freeze-thaw cycles, and keep the reagent briefly on ice during use to preserve antibody activity. The polyclonal nature of the reagent broadens epitope coverage beyond what a monoclonal typically offers, which is helpful when probing a conserved mitochondrial target in homogenates prepared for western blot analysis or in mitochondrial-enriched fractions.