Anti-MSH6 Antibody

Primary antibodies raised against the MSH6 mismatch repair protein are core reagents in oncology and genome stability research, supporting both clinical screening and mechanistic studies of DNA repair. This unconjugated rabbit polyclonal reagent from Antibodies.com is supplied as 100 µl of antiserum and has been validated for western blot, immunohistochemistry, and immunofluorescence workflows. The antibody recognizes human MSH6 (MutS Homolog 6) and cross-reacts with the mouse and rat orthologs, which makes it practical for translational projects that move between patient specimens and corresponding animal models.

MSH6 operates as the mismatch-recognition subunit of the MutSα heterodimer, pairing with MSH2 to scan newly replicated DNA for base-base mismatches and small insertion-deletion loops that escape polymerase proofreading. Loss of MSH6 function, through germline mutation in Lynch syndrome or through somatic silencing in sporadic tumors, destabilizes microsatellites and drives the microsatellite-instability-high phenotype observed in a meaningful subset of colorectal, endometrial, and gastric cancers. Researchers therefore use this reagent to profile mismatch repair status in formalin-fixed paraffin-embedded biopsies, to stratify tumors for immune checkpoint inhibitor responsiveness, and to investigate acquired resistance mechanisms tied to defective repair.

In immunohistochemistry, the antibody produces a clean nuclear signal in MMR-proficient epithelium and serves as one component of the standard four-protein panel read alongside MSH2, MLH1, and PMS2. On western blot, it detects a band near the expected 136 kDa molecular weight in lysates from repair-proficient lines such as HeLa, with absence or marked reduction confirmed in MSH6-deficient controls including the HCT116 background. Each vial is intended for single-use aliquoting to limit freeze-thaw cycles, with long-term storage recommended at -20 °C to preserve affinity across repeated experiments.