Anti-MSH5 Antibody

The MutS Homolog 5 (MSH5) protein operates as part of the DNA mismatch repair family and partners with MSH4 to form a heterodimer that drives crossover formation during meiotic recombination. Loss-of-function studies link MSH5 deficiency to meiotic arrest, reduced chiasma frequency, and associations with male infertility and certain lymphoma susceptibility loci, making antibodies against this target useful across reproductive biology and DNA damage response research.

This rabbit polyclonal antibody from Antibodies.com was raised against MSH5 and is validated for Western blot (WB) and immunohistochemistry (IHC) workflows. It detects both human and mouse orthologs, supporting comparative studies in model systems. The reagent is supplied unconjugated in a 100 µl vial, which gives researchers flexibility to pair it with HRP, biotin, or fluorophore chemistries of their choice and to scale assays from small pilot experiments to larger screening panels.

• Host species: Rabbit; polyclonal clonality for broad epitope coverage along the MSH5 protein
• Validated applications: Western blot and immunohistochemistry
• Species reactivity: Human and mouse
• Format: Unconjugated, liquid; 100 µl vial
• Target: MSH5 (MutS Homolog 5)

Typical readouts include probing testis or ovary lysates for MSH5 expression during prophase I, mapping MSH5 localization on meiotic chromosomes by IHC, and pairing the reagent with co-immunoprecipitation of MSH4 to confirm heterodimer assembly. Store refrigerated at 2 to 8 degrees C in single-use aliquots to limit freeze-thaw cycles of the polyclonal stock. For research use only.