Anti-MSH4 Antibody

From Antibodies.com, this rabbit polyclonal antibody is raised against MSH4 (mutS homolog 4) and supplied as an unconjugated liquid reagent of the IgG isotype. The catalogue entry lists validation for Western Blot and Immunofluorescence on human, mouse, and rat samples.

Manufacturer data for the corresponding reagent specifies Western Blot use with mouse and rat samples, with a recommended dilution of 1:500–1:2,000. The immunogen is a recombinant fusion protein corresponding to amino acids 697–936 of human MSH4 (NP_002431.2), and the target protein has a reported molecular weight of 105 kDa. The antibody is affinity-purified and supplied in phosphate buffered saline (pH 7.3) containing 50% glycerol and 0.02% sodium azide. It is shipped at 4°C; upon delivery, aliquot and store at −20°C and avoid freeze/thaw cycles.

Other Anti-MSH4 rabbit polyclonal reagents from the manufacturer cover additional applications. One variant is validated for Immunohistochemistry on human tissue at 1.3 mg/mL and detects endogenous levels of total MSH4 protein, supplied in PBS (pH 7.3) with 0.05% sodium azide and 50% glycerol. Another is raised against a synthetic peptide from amino acid region 700–800 of the Arabidopsis thaliana MSH4 gene and is validated for ELISA (1:10,000) and Western Blot (1:500), supplied at 0.95–1.04 µg/µL in a proprietary buffer (pH 7.4–7.8) containing 30% glycerol and 0.5% BSA.