Anti-MSH3 Antibody

Raised in rabbit against the human MutS Homolog 3 (MSH3) DNA mismatch repair protein, this unconjugated polyclonal IgG from Antibodies.com is supplied at 1 mg/ml in PBS with 0.1% Sodium Azide and 50% Glycerol, and is validated for Western blot (WB) and immunohistochemistry (IHC) on human samples. Each vial delivers 50 µl of working stock, suitable for several membrane-based and tissue-section staining experiments. MSH3 partners with MSH2 to form the MutSβ complex that recognizes and initiates repair of base-base mismatches and small insertion-deletion loops, making this reagent directly relevant to studies of microsatellite instability, Lynch syndrome (HNPCC), and tumor DNA damage response biology. IHC workflows on formalin-fixed paraffin-embedded (FFPE) tissue benefit from citrate-based heat-induced epitope retrieval and an anti-rabbit HRP-conjugated secondary, while WB applications on whole-cell lysates typically resolve a clear band above 110 kDa. Store at -20 °C in working aliquots to limit freeze-thaw cycles of the glycerol-containing formulation.