Anti-MSH3 Antibody

Raised in rabbit against the MSH3 (MutS Homolog 3) protein, this unconjugated polyclonal IgG reagent from Antibodies.com is supplied in a 200 µl vial and validated for Western blot and immunohistochemistry workflows on human, mouse, and rat samples. The antibody detects the 127 kDa mismatch repair protein that heterodimerizes with MSH2 to form MutSβ, the complex responsible for recognizing insertion-deletion loops formed during post-replicative DNA repair.

Researchers apply it to profile mismatch repair activity in tumor cell lines, characterize microsatellite instability in colorectal and endometrial cancers, and investigate EMAST (elevated microsatellite alterations at selected tetranucleotide repeats), a genome instability signature linked to a reversible nuclear-to-cytosolic shift of MSH3 driven by oxidative stress and IL-6 signaling. Pairing with HRP- or fluorophore-conjugated secondary reagents enables chemiluminescent or fluorescence detection, and immunohistochemistry on formalin-fixed paraffin-embedded tissue is typically performed with heat-induced epitope retrieval at working dilutions in the 1:50–1:200 range.

For best results, aliquot upon arrival, store refrigerated at 4°C, and limit freeze-thaw cycles to preserve binding activity. This product is for research use only and is not intended for diagnostic or therapeutic procedures.