Anti-mOrange Antibody

This rabbit polyclonal antibody from Antibodies.com is raised against mOrange, a red-shifted fluorescent protein widely used as a molecular tag for tracking fusion proteins in live cells and fixed samples. The unconjugated reagent is supplied as 100 µl of polyclonal serum and supports multiple detection workflows, including Western blot detection of mOrange-tagged constructs, immunofluorescence localization, immunoprecipitation of mOrange fusion complexes, and immunohistochemistry on tissue sections expressing the tag.

The polyclonal format allows recognition of multiple epitopes on the mOrange β-barrel, which is helpful when studying tagged fusion proteins where a single monoclonal epitope might be masked by a fused partner domain. Because the antibody targets mOrange itself rather than a generic red fluorescent protein consensus, it is best suited for experiments in which mOrange (or a closely matched mOrange2 derivative) is the specific reporter. Researchers working with related red fluorescent proteins such as mCherry, DsRed, or mScarlet should validate cross-reactivity individually before relying on it for those targets.

The unconjugated format gives users flexibility to pair the primary reagent with custom fluorophore or enzyme conjugates, including HRP, biotin, or Alexa Fluor secondaries, depending on whether the downstream read-out is chromogenic, fluorescent, or bead-based. Storage conditions and recommended working dilutions should be confirmed from the lot-specific data sheet shipped with the vial.