Anti-MMP-16 Antibody

This polyclonal reagent from Antibodies.com targets MMP-16 (also called MT3-MMP), a membrane-anchored matrix metalloproteinase implicated in extracellular matrix turnover, pro-MMP-2 activation, and tumor cell invasion pathways. Supplied as 100 µl of unconjugated antiserum raised in rabbit, the antibody is validated for Western blot and immunohistochemistry workflows across human, mouse, and rat sample types.

For Western blot applications, the reagent detects endogenous MMP-16 in cell lysates prepared from adherent or suspension cultures, resolving in the 50-70 kDa region depending on glycosylation state. In IHC, the antibody can be applied to formalin-fixed paraffin-embedded sections following citrate-based antigen retrieval, supporting studies of MMP-16 distribution in tumor microenvironments, synovial tissue, and developing organs where MT3-MMP expression has been linked to invasive behavior.

Researchers studying cancer cell migration, angiogenesis, and connective tissue remodeling have used MMP-16-directed antibodies to probe activation of downstream MMP-2 and to characterize pericellular proteolysis. The unconjugated format allows flexible pairing with HRP-, biotin-, or fluorophore-conjugated secondary reagents to suit chromogenic, fluorescence, or amplified detection systems.

Shipped as a 100 µl vial, the reagent is stored refrigerated between uses and is intended for research use only. Each lot is quality-controlled by Antibodies.com to confirm reactivity with the intended target, providing a consistent tool for laboratories investigating metalloproteinase biology.