Anti-MMP-13 Antibody

Raised in rabbit against matrix metalloproteinase 13 (MMP-13, also known as collagenase-3), this polyclonal antibody from Antibodies.com binds the active enzyme and pro-form zymogen expressed in articular cartilage, bone, and invasive tumor tissue. The unconjugated reagent is supplied as a 100 µl aliquot at 1 mg/ml in PBS buffer containing 0.1% Sodium Azide and 50% Glycerol, supporting stable storage at standard refrigerator conditions.

Validated applications include Western blot for protein-level confirmation across cell culture lysates and tissue homogenates, and immunohistochemistry for spatial mapping of MMP-13 expression in paraffin-embedded sections. Confirmed cross-reactivity covers human, mouse, and rat samples, enabling comparative work across osteoarthritis models, rheumatoid arthritis studies, and tumor microenvironment research focused on MMP-13-driven invasion and metastatic spread.

Store the vial at -20°C in aliquots to limit repeated freeze-thaw cycles, and protect from prolonged light exposure during handling. The polyclonal host response produces recognition of multiple epitopes on the MMP-13 catalytic and hemopexin-like domains, which can improve signal detection in denatured lysates as well as native tissue preparations.