Anti-MMP-11 Antibody

MMP-11 (matrix metalloproteinase 11), also known as stromelysin-3, is a zinc-dependent endopeptidase implicated in extracellular matrix remodeling, tumor progression, and tissue invasion. Unlike most secreted MMPs, MMP-11 is rarely detected in healthy adult tissues but becomes upregulated in carcinomas and in tumor-associated stromal compartments, where its expression is frequently linked to aggressive disease.

This rabbit polyclonal antibody from Antibodies.com was raised against human MMP-11 and cross-reacts with the mouse and rat orthologs, enabling comparative work across rodent tumor models and human surgical specimens. The unconjugated reagent is supplied as 100 µL of a 1 mg/mL stock in PBS containing 0.1% sodium azide and 50% glycerol, a buffer combination that supports storage at -20 °C and resists freeze-thaw damage for working aliquots.

Validated applications include Western blot for resolving pro- and processed MMP-11 forms in cell lysates and tumor extracts, and immunohistochemistry for localizing MMP-11 in formalin-fixed paraffin-embedded tissue sections. Investigators use these readouts to profile MMP-11 in breast, colorectal, and head-and-neck cancers, where stromal MMP-11 levels correlate with invasion and unfavorable prognosis.

Because the reagent is polyclonal, it binds multiple epitopes on MMP-11, improving detection of both full-length and catalytically active cleaved forms in denatured Western blot samples. The unconjugated format leaves secondary label selection open, allowing fluorescence, chromogenic, or chemiluminescent detection chemistries to be paired with the primary antibody.