Anti-MLH1 Antibody

MLH1 (MutL Homolog 1) is a central component of the cellular DNA mismatch repair machinery, and loss of its expression is a defining feature of mismatch repair-deficient tumors, particularly in colorectal and endometrial cancer research. This unconjugated mouse monoclonal reagent from Antibodies.com is raised against the human MLH1 protein and is validated for western blot (WB) and immunocytochemistry (ICC) workflows. Each vial contains 100 µl of purified immunoglobulin formulated in 0.1 M Tris-Glycine buffer (pH 7.4) supplemented with 150 mM NaCl, 0.2% sodium azide as preservative, and 50% glycerol for stable freezer storage. Investigators routinely apply this reagent to probe MLH1 protein levels in cultured human cell lines, characterize microsatellite instability phenotypes, and explore Lynch syndrome-associated signaling pathways. The product is supplied in a format that supports single-use aliquoting, permitting repeated freeze-thaw cycles without compromising antigen-binding performance in downstream assays.