Anti-MKP-1 / 2 Antibody

Raised in rabbit and affinity-purified as a polyclonal reagent, this unconjugated antibody recognizes MKP-1 (dual-specificity phosphatase 1, DUSP1) and the closely related MKP-2 (DUSP4). Both targets are inducible nuclear and cytosolic phosphatases that dephosphorylate activated MAPK family kinases such as ERK, JNK, and p38, which is why they are widely studied in inflammation, stress-response, and cell-signalling research.

The reagent is validated for western blot, immunohistochemistry, and ELISA workflows and cross-reacts with human, mouse, and rat samples, letting a single lot cover most common laboratory models. Supplied as 100 µl of unconjugated antisera, it is compatible with standard HRP, fluorescent, or biotin secondary labelling depending on the chosen application. The polyclonal format gives broader epitope coverage than a monoclonal reagent, an advantage when mapping expression changes or working with orthologous proteins that diverge at single residues.

Researchers using this Antibodies.com antibody typically probe whole-cell or tissue lysates after induction with cytokines, LPS, oxidative stress, or glucocorticoids, then visualise band shifts on western blot or staining patterns in fixed tissue sections. Aliquot on first use and store refrigerated to limit freeze-thaw cycles and preserve titre across extended studies.