Anti-MKK3 (Ab-189) Antibody

Manufactured by Antibodies.com, this rabbit polyclonal reagent is raised against a synthetic peptide spanning residues 187–191 (V-D-S-V-A) of human MKK3 (MAP2K3), enabling selective detection of the endogenous, total MKK3 protein in lysates and tissue sections. The antibody is supplied at 1.0 mg/mL in a 50 µL volume as an unconjugated IgG preparation in phosphate-buffered saline (pH 7.4) containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol, with no magnesium or calcium salts. It is validated for Western blotting (recommended dilution 1:500–1:1000), immunohistochemistry (1:50–1:100), and immunofluorescence (1:100–1:200), and the predicted molecular weight of the detected target is approximately 40 kDa. The host species is rabbit, the clonality is polyclonal, and the conjugate is unconjugated, allowing researchers to apply their own secondary labelling systems. Reactivity has been confirmed in human and mouse samples, consistent with the conservation of the immunogenic epitope around the Ab-189 site. For routine handling, the vial should be stored at -20°C after aliquoting upon arrival, with repeated freeze/thaw cycles avoided to preserve antibody integrity; the product is shipped at 4°C and is intended for research use only, not for diagnostic or therapeutic applications. Antibodies were purified by affinity chromatography using the epitope-specific peptide, supporting low-background performance across the listed applications.