Anti-MITF (phospho-S180) Antibody

This polyclonal phospho-specific antibody from Antibodies.com selectively recognizes the phosphorylated form of microphthalmia-associated transcription factor (MITF) at serine residue 180, supporting research into melanin biosynthesis regulation and melanocyte lineage signaling. Raised in rabbit and affinity purified, the reagent is supplied at 1 mg/ml in phosphate buffered saline (pH 7.2) containing 0.05% sodium azide as a preservative, with a 100 µl pack size accommodating several western blot and immunohistochemistry experiments.

MITF functions as a master transcriptional regulator in melanocyte differentiation, osteoclastogenesis, and mast cell development, driving expression of pigment enzymes such as tyrosinase, TYRP1, and DCT. Phosphorylation modulates its transactivation activity and subcellular distribution, making the antibody useful for studies of melanogenesis, melanoma biology, and pigment cell disorders. The reagent shows reactivity with human, mouse, and rat samples and is provided unconjugated for flexible assay design, including immunoblotting of phosphorylated MITF in cell lysates and immunohistochemical localization in tissue sections.

For best results, aliquot the antibody upon receipt, store refrigerated, and protect from light; avoid repeated freeze-thaw cycles. Suggested working ranges are approximately 1:500 to 1:2000 for western blotting and 1:50 to 1:200 for IHC, depending on antigen retrieval and sample preparation. This primary antibody is intended for research use only and supports investigations into Waardenburg syndrome type 2A, Tietz syndrome, and related melanocyte-related pathologies.