Anti-MITF Antibody

This polyclonal antibody from Antibodies.com targets Microphthalmia-Associated Transcription Factor (MITF), a basic helix-loop-helix leucine zipper transcription factor that acts as a master regulator of melanocyte development, pigmentation, and survival. Supplied as 100 µL of unconjugated rabbit antiserum, the reagent is validated for Western blot, immunohistochemistry, immunofluorescence, and ELISA workflows on human and mouse samples, giving laboratories a flexible reagent for both research pathology and basic melanocyte biology studies.

MITF is widely used in dermatopathology and oncology as a lineage marker for melanocytic lesions, where it drives melanogenesis through transcriptional control of tyrosinase, TYRP1, and DCT. Researchers working with melanoma cell lines, nevus biopsies, or pigment cell differentiation rely on this target to distinguish melanocytic identity from other neural crest derivatives. The polyclonal format allows recognition of multiple epitopes across the protein, which is useful when probing splice variants such as the M-isoform that predominates in melanocytes and melanoma cells.

Because the antibody is unconjugated, it pairs with a broad selection of secondary reagents, including HRP, biotin, Alexa Fluor, and other reporter systems chosen by the user. Storage at -20 °C in working aliquots is recommended to preserve activity across freeze-thaw cycles. Each lot is affinity purified and quality controlled, supporting reproducible band detection in Western blot and clear nuclear staining patterns in IHC and immunofluorescence of pigmented tissues and melanocytic tumors.