Anti-MITF Antibody

Microphthalmia-associated transcription factor (MITF) is a basic helix-loop-helix leucine zipper transcription factor that governs melanocyte lineage commitment, pigmentation gene expression, and survival of melanocytic cells. This unconjugated rabbit polyclonal antibody, supplied by Antibodies.com, was raised against MITF and recognises an epitope conserved across human, mouse, and rat orthologues, making it a useful reagent for comparative melanogenesis and melanoma studies. The antibody is formulated at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol as a cryoprotectant, and is shipped as a 100 µl vial ready for direct dilution into standard western blot buffers. Recommended storage is at -20°C in small aliquots to limit freeze-thaw cycles and preserve binding activity. Researchers commonly apply the reagent to western blot analysis of B16, MNT-1, SK-MEL-28, or MITF-transfected HEK293 lysates to confirm expression of full-length MITF or isoform-specific bands, and the polyclonal format increases the likelihood of detecting splice isoforms such as MITF-M and MITF-A. Because 0.1% sodium azide is included as a preservative, the reagent is well suited to western blot workflows but should be dialysed out or avoided when live-cell immunostaining is planned downstream. Each lot is quality controlled for reactivity against the three listed species, and the unconjugated format leaves the antibody free for direct coupling to HRP, biotin, or fluorescent dyes in custom assay development, supporting research use only applications in pigmentation, melanoma signalling, and melanocyte differentiation studies.