Anti-MIF Antibody

MIF (Macrophage Migration Inhibitory Factor) is a pro-inflammatory cytokine with a central role in innate immune signaling, where it is released from macrophages, T cells, and pituitary cells in response to infection, stress, and glucocorticoid counter-regulation. This polyclonal antibody from Antibodies.com is raised in rabbit and supplied as an unconjugated 100 µl reagent, validated for Western blot (WB) and immunohistochemistry (IHC) workflows on human, mouse, and rat samples. MIF's involvement in leukocyte recruitment, angiogenesis, and tumor progression makes it a frequent target in research on rheumatoid arthritis, sepsis, atherosclerosis, and oncogenic signaling pathways. Researchers commonly deploy this reagent to confirm endogenous MIF expression across cell lines and primary tissues, to study upstream regulation by NF-κB and HIF-1α, and to map MIF localization in fixed tissue sections. The polyclonal format increases the likelihood of recognizing multiple epitopes of the 12.5 kDa homotrimeric cytokine, which is helpful when probing denatured lysates or formalin-fixed paraffin-embedded specimens.