Anti-MIF Antibody

This rabbit polyclonal antibody from Antibodies.com targets Macrophage Migration Inhibitory Factor (MIF), a pleiotropic pro-inflammatory cytokine implicated in innate immune regulation, leukocyte recruitment, and the glucocorticoid counter-regulatory response to inflammation. The unconjugated reagent is supplied at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, the latter acting as a cryoprotectant that helps preserve antibody activity during frozen storage, in a 100 µl vial suitable for routine laboratory use.

Validated for western blot (WB) and immunohistochemistry (IHC) applications, the reagent recognizes the MIF target in human, mouse, and rat samples, supporting cross-species investigations in inflammatory disease models, oncology research, and studies of cytokine-driven signaling pathways. The glycerol-containing formulation is compatible with storage at -20 °C with minimal freeze-thaw cycling, while sodium azide protects against microbial contamination during repeated use at 4 °C working aliquots.

As a polyclonal reagent, the antibody binds multiple epitopes of MIF, which can be advantageous for detecting the cytokine across varied expression levels and post-translational contexts. Researchers commonly apply this reagent to examine MIF distribution in tissue sections by IHC and to probe cell or tissue lysates by western blot, making it a versatile tool for studies of inflammatory and immunomodulatory mechanisms.