Anti-MIEN1 Antibody

This polyclonal reagent from Antibodies.com targets MIEN1 (Migration and Invasion Enhancer 1), a membrane-anchored signaling protein involved in actin cytoskeleton remodeling, cell motility, and tumor cell invasion pathways. The antibody is supplied in a 100 µl vial at 1 mg/ml in phosphate buffered saline (PBS) containing 15 mM sodium azide at approximately pH 7.2.

Validated for Western blot application, the reagent detects MIEN1 of human, mouse, and rat origin, supporting translational studies comparing normal and malignant tissue lysates. MIEN1 sits within the chromosome 17q12 amplicon where it is frequently co-amplified with HER2 in breast tumors, and altered MIEN1 expression has been linked to prostate, oral, and gastrointestinal cancers in published research. Investigators probing migration and invasion phenotypes, prenylation of the CXXC motif, or downstream PI3K/Akt signaling in cancer cell models and xenograft tissue will find this reagent appropriate for in vitro studies.

As an unconjugated rabbit polyclonal preparation, the antibody can be paired with HRP- or fluorophore-conjugated anti-rabbit secondary reagents for chemiluminescent or fluorescent detection. Refrigerate working aliquots; avoid repeated freeze-thaw cycles and protect from light to preserve reagent integrity.