Anti-MGMT Antibody

Raised in rabbit against O-6-methylguanine-DNA methyltransferase, this polyclonal primary antibody from Antibodies.com targets the DNA repair enzyme responsible for directly reversing O6-alkylguanine adducts and safeguarding genomic integrity against alkylating damage. Validated for Western blot and immunofluorescence workflows, the unconjugated reagent detects endogenous MGMT across human, mouse, and rat samples, supporting comparative studies in translational oncology and neurobiology laboratories.

MGMT expression and promoter methylation status are central readouts in glioblastoma research, where silencing predicts responsiveness to temozolomide, and the marker is widely examined in colorectal, lung, and head-and-neck cancer models investigating alkylator resistance. The 100 µL format provides enough volume for multiple blotting membranes or IF coverslip preparations, and the polyclonal format offers broad epitope coverage for capturing the full-length ~21 kDa protein in whole-cell lysates and fixed-cell imaging.

Each lot is affinity-purified and supplied as a liquid aliquot that can be stored refrigerated for short-term use or frozen in working aliquots to limit freeze-thaw cycles. Researchers studying DNA repair pathways, glioma biomarker panels, or chemoresistance mechanisms can pair this reagent with a labelled secondary antibody for chemiluminescent or fluorescent detection without further conjugation steps.