Anti-METTL13 Antibody

Rabbit polyclonal antibody raised against METTL13, a protein methyltransferase best known for catalyzing trimethylation of eukaryotic elongation factor 1-alpha (eEF1A) at Lys55 and for installing N6-methyladenosine marks on 18S rRNA, both of which support ribosome biogenesis and translational fidelity. This unconjugated reagent from Antibodies.com is supplied at 1 mg/ml in PBS containing 0.1% sodium azide and 50% glycerol, and is shipped as a 100 µl vial that has been validated for western blot, immunohistochemistry, and immunofluorescence workflows on human samples and is reactive with METTL13 expressed in standard human cell lines.

Researchers studying oncogenic translation, eEF1A signaling, or ribosomal modification pathways commonly use this reagent to probe endogenous METTL13 levels and to monitor loss-of-function phenotypes following siRNA or CRISPR-mediated knockdown, where reduced methyltransferase activity slows proliferation and impairs cap-dependent protein synthesis. The polyclonal format offers broad epitope coverage, which is useful when validating antibody specificity by parallel western blot and IHC staining of matched tumor and normal tissue panels.

Store the vial at -20 °C in small aliquots to limit freeze-thaw cycles, and protect from light because the conjugate-free formulation relies on glycerol for stabilization. For research use only.