Anti-Methylphenidate Antibody

Manufactured by Antibodies.com as a high-purity research reagent, this Rabbit polyclonal immunoglobulin is raised against the small-molecule stimulant Methylphenidate (MPD, also known as Ritalin or MPH) and is intended for the development of competitive immunoassays. The immunogen is a Methylphenidate–BSA (Methylphenidate-BgG) conjugate, which makes the reagent well suited to detecting free drug in solution or on the solid phase of an ELISA plate. The antibody is supplied as a Protein A–purified polyclonal at a 1 mg pack size, presented in 0.05 M phosphate buffer (pH 7.15) containing 0.85 % sodium chloride and 0.05 % sodium azide as preservative, in a clear liquid format that is unconjugated and free of reporter enzymes or fluorophores. The host species is Rabbit, the clonality is polyclonal (IgG), and the conjugate label is none, allowing end users to couple it directly to HRP, biotin, or a fluorophore of their choice. Validated application is ELISA, and the antibody has been used to detect Methylphenidate in a competitive format in which 100 ng/mL analyte produces approximately 91 % inhibition. For short-term storage the vial should be kept refrigerated at +2 °C to +8 °C; for longer term, aliquot and store at ≤ –20 °C, avoiding repeated freeze/thaw cycles and protecting the product from light. The reagent is sold for Research Use Only and is supported by Antibodies.com's standard quality guarantee.