Anti-Metalloproteinase inhibitor 3 Antibody

The TIMP-3 protein (Metalloproteinase inhibitor 3) is a secreted glycoprotein central to extracellular matrix homeostasis, making it a focal target in research on tissue remodeling, fibrosis, and tumor microenvironment biology. This unconjugated rabbit polyclonal antibody from Antibodies.com is raised against the human TIMP-3 sequence and supplied as a 100 µg vial.

Validated for Western blot and immunohistochemistry, it enables detection of endogenous Metalloproteinase inhibitor 3 in cultured human cells, whole-cell lysates, and formalin-fixed tissue sections. TIMP-3 stands out among the four human TIMP family members because it anchors to the extracellular matrix via its C-terminal domain rather than diffusing freely, and it broadly suppresses matrix metalloproteinases (MMP-1, MMP-2, MMP-3, MMP-7, MMP-9) along with several ADAM and ADAMTS family proteases. Investigators studying angiogenesis, arthritis, chronic kidney disease, and solid tumors use TIMP-3 immunostaining to map pericellular protease balance.

• Host species: Rabbit
• Clonality: Polyclonal
• Conjugate: Unconjugated
• Validated applications: WB, IHC
• Reactivity: Human, with no significant cross-reactivity to the closely related TIMP-1 and TIMP-2 paralogs under standard assay conditions
• Format: 100 µg per vial

The polyclonal format provides broader epitope coverage than a monoclonal reagent, which is useful when probing denatured targets on Western blot membranes. For long-term storage, keep the vial refrigerated and avoid repeated freeze-thaw cycles by preparing single-use aliquots. For research use only.