Anti-MET (phospho Y1234 + Y1235) Antibody

Antibodies.com offers a rabbit polyclonal antibody raised against the activated form of the MET receptor tyrosine kinase, specifically recognizing phosphorylation at tyrosines 1234 and 1235 within the kinase activation loop. This site-specific reagent allows researchers to monitor ligand-induced MET signaling, since phosphorylation of Y1234/Y1235 is required for full catalytic activity of the receptor downstream of HGF binding.

The antibody is supplied as 50 µl of unconjugated IgG and is validated in Western blot workflows, where it produces a band at the expected molecular weight for MET (~150 kDa precursor / ~145 kDa mature β-chain after activation). Cross-reactivity has been demonstrated against human, mouse, and rat samples, making the reagent suitable for translational oncology programs, cell-line stimulation studies, and rodent models that probe invasive growth and scatter-factor signaling.

For best results, aliquot upon first thaw to limit freeze-thaw cycles and store the vial at -20 °C in a frost-free freezer. The polyclonal format provides tolerance for minor sequence variation around the phospho-epitope, and the unconjugated presentation leaves the user free to choose HRP, biotin, or fluorophore secondaries depending on the detection platform.