Anti-MET (phospho Y1234 + Y1235) Antibody

Raised in rabbit and supplied as an unconjugated IgG, this polyclonal phospho-specific reagent from Antibodies.com selectively recognizes the MET receptor tyrosine kinase only when it carries activating autophosphorylation marks at tyrosine residues 1234 and 1235 within the kinase activation loop. By Western blot, it resolves the activated ~145 kDa MET beta chain in lysates from ligand-stimulated cells, making it well suited to monitoring HGF/MET axis activation, receptor downregulation kinetics, and inhibition by small-molecule kinase inhibitors in oncology and receptor-biology workflows. Validated reactivity on Human, Mouse, and Rat samples allows phosphorylation studies across cell-line models and primary tissue lysates, supporting cross-species comparison of MET signaling. Each vial ships at 100 µl of ready-to-use reagent and should be stored refrigerated for routine working aliquots; for longer-term use, keep frozen and limit freeze-thaw cycles to preserve the phospho-epitope binding activity that defines this reagent's specificity.