Anti-MET (phospho Y1234) Antibody

Generated in rabbit as an IgG polyclonal reagent, this phospho-specific antibody from Antibodies.com selectively recognizes the MET receptor tyrosine kinase only when its activation-loop tyrosine 1234 is phosphorylated. Because Y1234 sits within the kinase domain whose autophosphorylation is triggered by HGF binding, the reagent provides a direct read-out of catalytically engaged MET rather than total receptor protein, which is critical when quantifying ligand-driven pathway activation in cell-based experiments.

Supplied unconjugated in a 50 µl vial, the antibody is validated for Western blot and immunofluorescence across human, mouse, and rat samples. Documented research uses include monitoring HGF-stimulated MET phosphorylation in hepatocellular carcinoma, non-small cell lung cancer, and gastric cancer models, tracking acquired MET amplification as a bypass mechanism in EGFR inhibitor-resistant tumors, and following kinase reactivation in time-course drug response studies. Investigators commonly pair it with a phosphorylation-independent MET antibody to derive phospho-to-total ratios and to confirm equal loading across treatment conditions.

The reagent is formulated in PBS with 50% glycerol and 0.02% sodium azide, shipped cold and intended for storage at -20 °C with aliquoting to avoid repeated freeze-thaw cycles. For research use only, the antibody supports studies of receptor tyrosine kinase dynamics, epithelial-mesenchymal transition, and downstream signaling cascades that depend on MET autophosphorylation at its primary activation site.