Anti-MET Antibody

Distributed by Antibodies.com as an antigen-affinity-purified rabbit polyclonal reagent in a 100 µl unconjugated liquid format, this antibody targets the MET receptor tyrosine kinase. The immunogen is a synthetic peptide derived from human MET, and the reagent is documented to detect endogenous levels of total MET protein across human, mouse, and rat samples. Supplied at 1.8 mg/mL in pH 7.4 PBS with 0.05% sodium azide and 40% glycerol, the formulation supports Western blot, immunohistochemistry, and immunocytochemistry workflows without the constraints of a pre-labeled secondary format.

Validation data on the product datasheet confirm Western blot performance using 40 µg of HepG2 cell lysate resolved on a 6% SDS-PAGE gel, with the primary antibody applied at a 1/200 dilution and a goat anti-rabbit IgG secondary at 1/8000 over a 10-minute exposure. Parallel immunohistochemistry validation is documented on paraffin-embedded human oesophagus cancer tissue at a 1/30 dilution, providing a reference for tumor-tissue localization studies in non-small cell lung cancer, hepatocellular carcinoma, and gastric carcinoma research, where aberrant MET signaling drives proliferation, scattering, and invasive growth through downstream PI3K/AKT, RAS/MAPK, and STAT pathways.

The unconjugated format enables pairing with custom fluorophore, biotin, or enzyme conjugates selected to fit a chosen detection chemistry, and is compatible with phospho-MET co-detection experiments that distinguish ligand-dependent and ligand-independent receptor activation. For handling, the vial ships cold and should be aliquoted into working volumes upon receipt and stored at -20°C, with short-term use acceptable at 4°C; repeated freeze-thaw cycles should be avoided to preserve binding activity. Each vial is intended for research use only and is not validated for diagnostic or therapeutic applications.