Anti-MEP50 Antibody

MEP50, the WD-repeat protein 77 (WDR77) subunit of the methylosome complex that partners with protein arginine methyltransferase 5 (PRMT5) to catalyze symmetric arginine dimethylation on substrate proteins, can be detected with this mouse monoclonal antibody from Antibodies.com.

Supplied as 100 µl of purified mouse IgG in a single vial containing 0.1 M Tris-Glycine buffer (pH 7.4), 150 mM NaCl, 0.2% sodium azide, and 50% glycerol, the unconjugated reagent is validated for Western blot and immunocytochemistry on human, mouse, and rat samples, supporting comparative work across species and cell lines.

Typical applications include probing cell lysates for endogenous MEP50 by Western blot and visualizing the protein in fixed cultured cells by immunocytochemistry, contributing to research on RNA splicing, snRNP biogenesis, and PRMT5-linked signaling pathways in vitro. For repeated use, the antibody should be aliquoted to limit freeze-thaw cycles.