Anti-MEOX1 Antibody

Raised in rabbit as an unconjugated polyclonal IgG, this Antibodies.com reagent is supplied as a 200 µl liquid preparation targeting human mesenchyme homeobox 1 (MEOX1). The antigen is a synthetic peptide from the central region of human MEOX1, and the resulting IgG fraction recognizes the homeodomain transcription factor that directs sclerotome polarity, somite compartmentalization, and cranio-cervical joint formation during vertebrate embryogenesis. The native MEOX1 protein runs at approximately 34 kDa on Western blot.

Cross-reactivity with the mouse and rat orthologues makes this antibody useful for comparative studies of axial skeletogenesis and limb musculature in mammalian model systems. Homozygous Meox1 loss-of-function in mice produces assimilation of the atlas into the basioccipital bone, a phenotype that mirrors the cranio-cervical fusion seen in Klippel-Feil syndrome, and the reagent is therefore used by groups working on congenital skeletal disorders. MEOX1 has also been mapped to evolutionarily conserved promoter regions of Tbx18, Uncx, and Bapx1, providing a chromatin-level readout for downstream transcription factor studies by chromatin immunoprecipitation.

Validated applications include Western blot for expression profiling in cell and tissue lysates, and immunofluorescence on fixed cells and cryosections to localize the transcription factor within the nucleus. The unconjugated format gives laboratories flexibility to pair the reagent with any HRP- or fluorophore-conjugated secondary chosen by the end user. The vial should be stored at -20 °C, remains stable for one year, and should be protected from repeated freeze-thaw cycles.