Anti-MELK Antibody

Validated for Western blotting (WB), this rabbit polyclonal primary antibody from Antibodies.com targets MELK. It is unconjugated and reacts with human, mouse, and rat samples. The immunogen is a peptide sequence around aa.626~630 (I-R-R-Q-R) derived from human MELK, and the antibody detects the endogenous level of total MELK protein.

The antibody is supplied at 1.0 mg/mL in phosphate-buffered saline, pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol. Its predicted molecular weight is 74kd. The recommended dilution for Western blotting is 1:500. It is shipped at 4°C; upon delivery, aliquot and store at -20°C, and avoid freeze/thaw cycles. Production involved immunizing rabbits with synthetic peptide and KLH conjugates, followed by purification using affinity chromatography with an epitope-specific peptide.