Anti-MEK2 (Ab-394) Antibody

A polyclonal reagent from Antibodies.com raised in rabbit against the MAP2K2 kinase (also known as MEK2), a dual-specificity threonine/tyrosine kinase that sits between RAF and ERK1/2 in the canonical RAS-RAF-MEK-ERK signal transduction cascade. The antibody is supplied as 100 µl of unconjugated Ig fraction, affinity-purified against the human MEK2 target, and cross-reacts with the rat ortholog, giving researchers a single reagent for comparative work across human cell lines and rodent tissue sections.

Validation has been performed for Western Blot (WB) and immunohistochemistry (IHC) workflows, supporting studies of mitogenic signaling, oncogenic pathway activation, and downstream ERK phosphorylation in fixed tissue and lysate preparations. MEK2 is frequently evaluated alongside MEK1 in cancer research on melanoma, colorectal, and thyroid tumors where small-molecule MAPK pathway inhibitors are being characterized for their effects on phosphorylated kinase signaling.

The unconjugated format pairs cleanly with standard HRP- or fluorophore-conjugated secondary antibodies compatible with rabbit IgG, and the reagent can be aliquoted and stored frozen for repeated use across multiple experiments.