Anti-MEK1 / MEK2 (Ab-217 / 221) Antibody

This rabbit polyclonal antibody from Antibodies.com is raised against the activating dual-phosphorylation motif of MEK1 and MEK2 (MAP2K1/MAP2K2), recognizing epitopes flanking threonine 217 and serine 221. Phosphorylation at these adjacent residues drives MEK catalytic activity within the canonical Raf/MEK/ERK signal transduction cascade, making the reagent useful for dissecting mitogenic signaling, oncogenic pathway activation, growth factor responses, and stress-induced MAPK regulation in mammalian cells.

Validated for Western blot, immunohistochemistry, and immunofluorescence, the unconjugated antibody is supplied as a 100 µL liquid aliquot and pairs with HRP, biotin, or fluorophore-conjugated secondary reagents depending on assay design. Cross-reactivity is confirmed with MEK1/MEK2 orthologs from human, mouse, and rat samples, supporting comparative studies in cultured cell lines, primary tissue sections, and rodent disease models.

Recommended applications include probing ERK pathway activation in tumor lysates, monitoring MAPK dynamics after small-molecule kinase inhibitor treatment, and characterizing phosphorylation changes in fixed tissue specimens. For best results, refrigerate short-term, aliquot to avoid repeated freeze-thaw cycles, and protect from light if a fluorescent workflow is planned downstream.