Anti-MEK1 Antibody

Supplied as 100 µl of unconjugated antibody at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, this rabbit polyclonal reagent from Antibodies.com is raised against MEK1 (MAP2K1), the dual-specificity kinase that activates ERK1/2 in the canonical RAS-RAF-MEK-ERK signaling cascade. Validated for western blot and immunohistochemistry, the antibody shows reactivity with human, mouse, and rat samples, supporting cross-species studies in cancer biology, signal transduction, and cell proliferation. MEK1 is a frequent subject of oncology research because activating mutations in MAP2K1, along with upstream BRAF and RAS alterations, drive constitutive MAPK pathway activation in melanoma, hairy cell leukemia, and Langerhans cell histiocytosis. Investigators use this reagent to probe pathway activation status, validate small-molecule MEK inhibitor responses, and assess ERK signaling in tumor and normal tissue sections. The polyclonal format recognizes multiple epitopes on MEK1, which can be advantageous when probing for variant or truncated forms relevant to certain disease models. Aliquot upon arrival and store at -20°C to preserve antibody activity across multiple freeze-thaw cycles; avoid repeated freeze-thaw cycles when possible. Sodium azide is included as a preservative; the 50% glycerol cryoprotectant allows storage at -20°C without freezing.