Anti-MEK1 (Ab-221) Antibody

MEK1 (also known as MAP2K1) is a dual-specificity kinase at the core of the RAS-RAF-MEK-ERK signal transduction cascade, phosphorylating ERK1 and ERK2 in response to growth factor and mitogenic cues. This affinity-purified rabbit polyclonal reagent from Antibodies.com was raised against a synthetic peptide corresponding to residue 221 of human MEK1, a region that overlaps a regulatory phosphorylation site within the kinase domain. The antibody is supplied as 100 µL of unconjugated antiserum and is validated for Western blot and immunohistochemistry applications on human and mouse samples.

Researchers working on cancer cell signaling, developmental biology, and neurobiology routinely use anti-MEK1 reagents to assess pathway activation in cell lysates and to localize MEK1 expression in fixed tissue sections. The polyclonal format increases the chance of detecting the target even when the protein is partially degraded during sample preparation. Each vial should be aliquoted upon first thaw to minimize freeze-thaw cycles and to preserve reagent performance across multiple experiments.