Anti-MEF2A (phospho T319) Antibody

This phospho-specific rabbit polyclonal antibody from Antibodies.com is raised against a synthetic phosphopeptide corresponding to the V-T-T(p)-P-S sequence surrounding threonine 319 of human MEF2A. The reagent selectively recognizes endogenous MEF2A only when that residue is phosphorylated, allowing direct tracking of MEF2A activation status in cells and tissues. Thr319 lies within the C-terminal transactivation domain of MEF2A together with Thr312, and p38 mitogen-activated protein kinase phosphorylates this pair of sites to potentiate MEF2-dependent gene expression during muscle differentiation, stress responses, and neuronal signaling.

Affinity purification is performed against the epitope-specific phosphopeptide, followed by a non-phosphopeptide subtraction step that removes antibodies cross-reactive with the unphosphorylated protein, supporting clean discrimination between phospho- and non-phospho MEF2A. The antibody is supplied unconjugated as a 100 µl liquid formulation in PBS at pH 7.4 with 150 mM NaCl, 0.02% sodium azide, and 50% glycerol, validated for immunofluorescence applications such as ICC and IF on fixed cells or tissue sections from human, mouse, or rat samples. Researchers can pair it with fluorophore- or biotin-conjugated secondary reagents matched to widefield, confocal, or high-content imaging platforms.

For handling, the vial is shipped at 4 °C; once received, it should be aliquoted and stored at -20 °C, and freeze/thaw cycles avoided to preserve activity across multiple experiments.