Anti-MED11 Antibody

The Mediator complex subunit 11 (MED11) is a small but essential component of the mammalian Mediator, a multi-subunit bridge that transmits regulatory cues from DNA-bound transcription factors to RNA polymerase II during eukaryotic gene expression. This rabbit polyclonal reagent from Antibodies.com was raised against MED11 and is validated for western blot detection of the endogenous protein in lysates prepared from human, mouse, and rat cells.

The antibody is supplied at 100 µl of an unconjugated preparation at 1 mg/ml in phosphate-buffered saline with 15 mM sodium azide at approximately pH 7.2, ready for use with secondary detection reagents of the researcher's choice. Polyclonal preparations typically recognize multiple epitopes on the target, which can be advantageous when probing denatured samples on western blots and supports detection across the listed mammalian species.

For best results, store refrigerated and aliquot to avoid repeated freeze-thaw cycles that can degrade antibody activity. Researchers studying transcriptional regulation, RNA polymerase II function, or the mechanistic role of Mediator in disease-associated signaling pathways will find this reagent useful for probing endogenous MED11 levels and its incorporation into Mediator subcomplexes.