Anti-MED1 Antibody

This rabbit polyclonal IgG reagent from Antibodies.com targets the N-terminal region (residues 1–300) of human Mediator Complex Subunit 1 (MED1, NP_004765.2), a 250 kDa nuclear protein that anchors ligand-activated transcription factors to RNA polymerase II. The antibody is affinity purified and supplied unconjugated in a 100 µl vial for western blot workflows.

In-house validation on MCF7 whole-cell lysates identifies a single band at the expected molecular weight, with 25 µg of total protein loaded per lane and detection achieved via ECL at a working dilution of 1:500 to 1:1,000. The immunogen sequence is conserved across human, mouse, and rat orthologs, supporting investigations of nuclear receptor signaling, adipogenesis, embryonic development, and enhancer–promoter looping in chromatin-based reporter assays and gene expression studies.

Storage and handling: keep the vial refrigerated between uses; for extended storage, aliquot and freeze at -20 °C, limiting freeze-thaw cycles. The reagent is intended for research use only and is not validated for diagnostic or therapeutic applications.