Anti-MDM2 (phospho S166) Antibody

Phosphorylation of MDM2 at serine 166 is a key regulatory event that modulates its E3 ubiquitin ligase activity and nuclear localization, influencing p53 suppression and downstream cell-cycle signaling. The Antibodies.com Anti-MDM2 (phospho S166) Antibody is a rabbit polyclonal IgG reagent raised against this modified epitope, supplied as 50 µl of unconjugated antiserum for use in western blot and immunofluorescence workflows. Validated reactivity across human, mouse, and rat samples makes it well suited for comparative oncology studies, DNA damage response assays, and signaling pathway research where p53-MDM2 feedback loops are under investigation. Researchers can apply the antibody directly to fixed cells for IF imaging of phospho-S166 nuclear foci or to lysate fractions for WB detection of the modified band, typically resolving above the unmodified MDM2 species on SDS-PAGE.