Anti-MDM2 (phospho S166) Antibody

Phosphorylation of MDM2 at serine 166 is a key regulatory event in the p53 tumor suppressor pathway, and this unconjugated rabbit polyclonal reagent from Antibodies.com is raised specifically against that modified site. Raised in rabbit and supplied at 200 µl, the IgG antibody is validated for Western blot, immunohistochemistry, and immunofluorescence workflows in human samples, supporting research into DNA damage signaling, Akt-mediated control of MDM2 activity, and downstream p53 stabilization.

Researchers studying the phosphorylation-dependent activation of MDM2 in cancer cell lines, tumor tissue sections, and fixed-cell preparations can use this reagent to track the spatial distribution and abundance of the activated form of MDM2 in response to genotoxic stress, growth factor signaling, or therapeutic intervention. The unconjugated format allows flexible pairing with secondary antibodies and fluorophores of the user's choice, which is helpful for multiplexed imaging experiments where phospho-MDM2 is examined alongside other pathway components.