Anti-MDM2 Antibody

This unconjugated polyclonal antibody from Antibodies.com targets MDM2 (Mouse Double Minute 2), the E3 ubiquitin-protein ligase that serves as the principal negative regulator of the p53 tumor suppressor pathway. Produced in rabbit and supplied as 100 µl of liquid, the reagent is validated for western blot, immunohistochemistry, and immunofluorescence workflows on human and rat samples.

MDM2 binds p53 through its N-terminal transactivation domain and ubiquitinates the tumor suppressor, targeting it for proteasomal degradation. Amplification or overexpression of MDM2 has been documented across multiple tumor types, making the protein a focal target in cancer biology research, drug discovery programs aimed at restoring p53 activity (including nutlin-class and ALRN-6924-class disruptors of the MDM2-p53 interaction), and studies of DNA damage and genomic stress responses.

Investigators apply the antibody to assess endogenous MDM2 expression, monitor p53 pathway status, and probe MDM2-containing protein complexes. Store at -20 °C in working aliquots to limit freeze-thaw cycles; the unconjugated format supports flexible pairing with secondary antibodies for chromogenic or fluorescent detection. Each vial is intended for research use only and is reactive with both human and rat MDM2.