Anti-MDM2 Antibody

This unconjugated polyclonal reagent from Antibodies.com is raised in rabbit and targets MDM2 (Mouse Double Minute 2), an E3 ubiquitin-protein ligase that serves as the principal negative regulator of the p53 tumor suppressor pathway. By ubiquitinating p53 and promoting its nuclear export and proteasomal degradation, MDM2 functions as a critical oncogenic driver whose amplification or overexpression is observed in numerous human cancers, making antibodies against it useful tools in oncology and cell-signalling research.

The antibody is validated for western blot, immunohistochemistry, immunofluorescence, and ELISA workflows, and has been tested for reactivity against human, mouse, and monkey samples. Supplied as 100 µl of unconjugated antiserum suitable for detection in standard immunostaining and blotting protocols, it provides flexibility for both chromogenic and fluorescence-based readouts depending on the secondary reagent chosen by the user.

Researchers studying p53 pathway biology, genotoxic stress responses, and tumourigenesis frequently rely on MDM2 detection to characterise transformed cell lines and tissue specimens. The polyclonal nature of the reagent allows recognition of multiple epitopes on the MDM2 protein, which can be advantageous when probing for variants or truncated isoforms of the target.