Anti-MDM2 Antibody

This unconjugated rabbit polyclonal antibody from Antibodies.com is raised against MDM2, the E3 ubiquitin-protein ligase that serves as the principal negative regulator of the p53 tumor suppressor pathway. Supplied at 1 mg/ml in phosphate buffered saline (PBS) with 0.05% sodium azide at approximately pH 7.2, the reagent is validated for western blot detection of endogenous MDM2 in samples from human, mouse, and rat origins.

MDM2 is a focal point in cancer biology research: its overexpression by gene amplification or transcriptional upregulation disables p53-mediated tumor suppression in many sarcomas, glioblastomas, and carcinomas. Investigators studying DNA damage signaling, cell cycle checkpoint control, and the pharmacology of small-molecule MDM2 antagonists such as the nutlin-class inhibitors use this reagent to monitor protein abundance, characterize drug responses in in vitro cell-based assays, and explore autoregulatory feedback with p53.

The 100 µl volume is supplied as a ready-to-dilute stock for western blot. Aliquot and store frozen at -20°C for long-term stability; avoid repeated freeze-thaw cycles. The 0.05% sodium azide serves as a preservative.